Emma K. NickersonVanaporn WuthiekanunVarun KumarPremjit AmornchaiNattavut WongdeethaiKheng ChhengNarisara ChantratitaHor PutchhatJanjira ThaipadungpanitNicholas P. DaySharon J. PeacockAddenbrooke's HospitalMahidol UniversityAngkor Hospital for ChildrenUniversity of Cambridge2018-05-032018-05-032011-02-01American Journal of Tropical Medicine and Hygiene. Vol.84, No.2 (2011), 313-317000296372-s2.0-79952642163https://repository.li.mahidol.ac.th/handle/123456789/12085We previously described the first reported isolation of methicillin-resistant Staphylococcus aureus (MRSA) (a case series of pediatric community-associated MRSA infections) in Cambodia. We define the rate of pediatric MRSA carriage in the same population and characterize the associated bacterial genotypes by using pulsed-field gel electrophoresis and multilocus sequence typing. A prospective cohort study of MRSA carriage conducted over one month at the Angkor Hospital for Children, Siem Reap, Cambodia, identified MRSA carriage in 87 (3.5%) of 2,485 children who came to the outpatient department, and 6 (4.1%) of 145 inpatients, including at least two with cases of nosocomial acquisition. Genotyping of all 93 MRSA isolates resolved 5 genotypes. Most (91%) isolates were assigned to sequence type 834. Only 28 (32%) of 87 MRSA carriers identified in the outpatient department had no history of recent healthcare contact. The study findings have important implications for healthcare in a setting where diagnostic microbiology and access to antimicrobial drugs with efficacy against MRSA are limited. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.2011.10-0300