Aviva GeretzPhilip K. EhrenbergAlain BouckenoogheMarcelo A. Fernández ViñaNelson L. MichaelDanaya ChansinghakuleKriengsak LimkittikulRasmi ThomasStanford University School of MedicineHJFWalter Reed Army Institute of ResearchMahidol UniversityAsia-Pacific Clinical DevelopmentAsia-Pacific Clinical Development2019-08-232019-08-232018-11-01Human Immunology. Vol.79, No.11 (2018), 773-78018791166019888592-s2.0-85054032786https://repository.li.mahidol.ac.th/handle/20.500.14594/45949© 2018 The human leukocyte antigen (HLA) genes are highly variable and are known to play an important role in disease outcomes, including infectious diseases. Prior knowledge of HLA polymorphisms in a population usually forms the basis for an effective case-control study design. As a prelude to future disease association analyses, we report HLA class I and II diversity in 334 unrelated donors from a Dengue vaccine efficacy trial conducted in Thailand. Long-range PCR amplification of six HLA loci was performed on DNA extracted from saliva samples. HLA-A, -B, -C, -DPB1, -DQB1 and -DRB1 were genotyped using a next-generation sequencing method presented at the 17th International HLA and Immunogenetics Workshop. In total, we identified 201 HLA alleles, including 35 HLA-A, 57 HLA-B, 28 HLA-C, 24 HLA-DPB1, 21 HLA-DQB1 and 36 HLA-DRB1 alleles. Very common HLA alleles with frequencies greater than 10 percent were A∗11:01:01, A∗33:03:01, A∗24:02:01, B∗46:01:01, C∗07:02:01, C∗01:02:01, C∗08:01:01, DPB1∗05:01:01, DPB1∗13:01:01, DPB1∗04:01:01, DPB1∗02:01:02, DQB1∗03:01:01, DQB1∗05:02:01, DQB1∗03:03:02, DRB1∗12:02:01, DRB1∗09:01:02, and DRB1∗15:02:01. A novel HLA allele, B∗15:450, had a non-synonymous substitution and occurred in more than one donor. Population-based full-length NGS HLA typing is more conclusive and provides a sound foundation for exploring disease association in a given population.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.humimm.2018.09.005