Kesinee ChotivanichKamolrat SilamutRachanee UdomsangpetchKatarzyna A. StepniewskaSasithon PukrittayakameeSornchai LooareesuwanNicholas J. WhiteMahidol UniversityNuffield Department of Clinical Medicine2018-09-072018-09-072001-01-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.95, No.6 (2001), 677-680003592032-s2.0-0035726301https://repository.li.mahidol.ac.th/handle/123456789/26588A simple reproducible method for short-term ex-vivo Plasmodium vivax culture is presented in which glucose, ascorbic acid, thiamine, hypoxanthine, and 50% human AB+serum are added to the standard P. falciparum in-vitro culture medium. Culture of freshly obtained blood samples from patients with acute vivax malaria with >0.5% parasitaemia resulted in >95% complete schizogony. Culture could be continued for 5-6 cycles without the addition of red cells. Criteria for staging the erythrocytic development of P. vivax in the first schizogonic cycle based on synchronous ex-vivo culture are presented. The asexual cycle was divided into 7 morphological stages: tiny ring (0-6 h), small ring (6-12 h), large ring (12-18 h), early trophozoite (18-28 h), late trophozoite (28-36 h), early schizont (36-42 h) and mature schizont (42-48 h). This simple method of culturing P. vivax ex vivo is suitable for antimalarial susceptibility and immunoparasitology studies.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/S0035-9203(01)90113-0