Anuchai NiwetpathomwatGalayanee DoungchaweeChulalongkorn UniversityMahidol University2018-08-202018-08-202006-03-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.37, No.2 (2006), 309-311012515622-s2.0-33746078045https://repository.li.mahidol.ac.th/handle/20.500.14594/23801Leptospirosis is a major zoonotic disease throughout the world. There are unavailable accuracy diagnostic methods for the acute phase of infection. To demonstrate the advantage of Western immunoblot, a mixed leptospira serovars antigen for the serodiagnosis of leptospirosis was employed. SDS-PAGE and Western immunoblot was performed using a 10 mixed leptospira serovars antigen and stained with 16 reference rabbit anti-leptospirosis antibodies. The result showed different immunoreactive band patterns for each reference serum. The bands with molecular weights of 15-20, 23-24, 41 and 45 kDa were commonly found (88% to 100% of the 16 reference sera). Using combined leptospira antigens in a Western immunoblot technique is an alternative and practical strategy for a more sensitive leptospirosis serodiagnosis.Mahidol UniversityMedicineArticleSCOPUS