Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor

Ekkachai KittiloespaisanAkio OjidaItaru HamachiYortyot Seetang-NunWansika KiatpathomchaiJirarut WongkongkatepMahidol UniversityKyushu UniversityKyoto UniversityThailand National Center for Genetic Engineering and Biotechnology2018-06-112018-06-112012-12-01Chemistry Letters. Vol.41, No.12 (2012), 1666-166813480715036670222-s2.0-84872230875https://repository.li.mahidol.ac.th/handle/20.500.14594/13932A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 12Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a highthroughput manner with high sensitivity and selectivity. © 2012 The Chemical Society of Japan.Mahidol UniversityChemistryLabel-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensorArticleSCOPUS10.1246/cl.2012.1666