Shambavi SubbaraoSuphak VanichseniDale J. HuDwip KitayapornKachit ChoopanyaSuwanee RakthamNancy L. YoungChantapong WasiRuengpung SutthentChi Cheng LuoArtur RamosTimothy D. MastroCenters for Disease Control and PreventionBangkok Metropolitan AdministrationMahidol UniversityHIV/AIDS CollaborationThailand Ministry of Public Health2018-09-072018-09-072000-05-20AIDS Research and Human Retroviruses. Vol.16, No.8 (2000), 699-707088922292-s2.0-0034690623https://repository.li.mahidol.ac.th/handle/20.500.14594/25985We obtained specimens from 128 HIV-1 seroconverters identified from 1995 through 1998 in a prospective cohort study of 1209 HIV-negative injecting drug users (IDUs) in Bangkok, Thailand. Epidemiologic data indicated that parenteral transmission accounted for nearly all infections. HIV-1 DNA from the C2-V4 env region was sequenced, and phylogenetic analyses determined that 102 (79.7%) of the specimens were subtype E and 26 (20.3%) subtype B strains. All subtype B strains clustered with strains often referred to in previous studies as Thai B or B'. The interstrain nucleotide distance (C2-V4) within subtype E strains was low (mean, 6.8%), and pairwise comparisons with a prototype subtype E strain, CM244, showed limited divergence (mean, 5.6%). The subtype B stains showed greater interstrain divergence (mean, 9.2%) and were significantly divergent from the prototype B strain HIV-MN (mean, 13.0%; p < 0.0001). The subtype E strains had significantly lower mean V3 loop charge than did subtype B strains (p = 0.017) and, on the basis of analysis of amino acid sequences, were predicted to be predominantly (91%) non- syncytium-inducing (NSI), chemokine coreceptor CCR5-using (CCR5+) viruses. The subtype B strains had a higher mean V3 loop charge, and a smaller proportion (23%) were predicted to be NSI/CCR5+ viruses. This study demonstrates that most incident HIV-1 infections among Bangkok IDUs are due to subtype E viruses, with a narrow spectrum of genetic diversity. The characterization of incident HIV-1 strains from 1995 to 1998 will provide important baseline information for comparison with any breakthrough infections that occur among IDUs in Bangkok who are participating in an HIV-1 vaccine efficacy trial initiated in 1999.Mahidol UniversityImmunology and MicrobiologyMedicineGenetic characterization of incident HIV type 1 subtype E and B strains from a prospective cohort of injecting drug users in Bangkok, ThailandArticleSCOPUS10.1089/088922200308693