Imerb N.Thonusin C.Pratchayasakul W.Arunsak B.Nawara W.Aeimlapa R.Charoenphandhu N.Chattipakorn N.Chattipakorn S.C.Mahidol University2023-06-182023-06-182022-04-15Life Sciences Vol.295 (2022)00243205https://repository.li.mahidol.ac.th/handle/20.500.14594/83758Aims: To investigate the effects of hyperbaric oxygen therapy (HBOT) on metabolic disturbance, aging and bone remodeling in D-galactose-induced aging rats with and without obesity by determining the metabolic parameters, aging and oxidative stress markers, bone turnover markers, bone microarchitecture, and bone biomechanical strength. Materials and methods: Male Wistar rats were fed either a normal diet (ND; n = 18) or a HFD (n = 12) for 22 weeks. At week 13, vehicle (0.9% NaCl) was injected into ND-fed rats (NDV; n = 6), while 150 mg/kg/day of D-galactose was injected into 12 ND-fed rats (NDD) and 12 HFD-fed rats (HFDD) for 10 weeks. At week 21, rats were treated with either sham (NDVS, NDDS, or HFDDS; n = 6/ group) or HBOT (NDDH, or HFDDH; n = 6/group) for 14 days. Rats were then euthanized. Blood samples, femora, and tibiae were collected. Key findings: Both NDD and HFDD groups developed aging as indicated by increased AGE level, increased inflammation and oxidative stress as shown by raised serum TNF-α and MDA levels, impaired bone remodeling as indicated by an increase in levels of CTX-1, TRACP-5b, and impaired bone structure/strength, when compared with those of the NDVS group. HFD aggravated these indicators of bone dyshomeostasis in D-galactose-treated rats. HBOT restored bone remodeling and bone structure/strength in the NDD group, however HBOT ameliorated bone dyshomeostasis in the HFDD group. Significance: HBOT is a potential intervention to decrease the risk of osteoporosis and bone fracture in aging with or without obesity.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.lfs.2022.1204062-s2.0-851247512611879063135182555