Paiboon VattanaviboonSkorn MongkolsukChulabhorn Research InstituteMahidol University2018-07-042018-07-041998-12-15FEMS Microbiology Letters. Vol.169, No.2 (1998), 255-260037810972-s2.0-0032466341https://repository.li.mahidol.ac.th/handle/123456789/18271Hydroxyl radical, a product of H2O2metabolism in the Fenton reaction, is a major reactive oxygen species involved in H2O2killing of Xanthomonas campestris pv. phaseoli (Xp). Compounds which absorbed hydroxyl radicals protected Xanthomonas from H2O2killing, but not from killing by a superoxide generator or an organic peroxide. Addition of iron potentiated H2O2killing. On the other hand, pre-treatment of Xp with an iron chelator showed no protective effects and H2O2killing was actually enhanced. Unexpectedly, resuspension of Xp in either water or phosphate buffer induced high-level resistance to H2O2killing. The unknown protection mechanism was independent of new protein synthesis. Copyright (C) 1998 Federation of European Microbiological Societies.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyEvaluation of the role hydroxyl radicals and iron play in hydrogen peroxide killing of Xanthomonas campestris pv. phaseoliArticleSCOPUS10.1016/S0378-1097(98)00494-7