Yongyuth YuthavongMahidol University2018-04-302018-04-301980-06-02Life Sciences. Vol.26, No.22 (1980), 1899-1903002432052-s2.0-0019314151https://repository.li.mahidol.ac.th/handle/20.500.14594/11187Pronase treatment of mouse red cells in the presence of chloroquine leads to greatly enhanced accumulation of the drug, which after freeze-thaw or hypotonic lysis is found to be located mainly in the membrane fraction. Much lower proportions of the drug are found in the membrane fraction prepared from Plasmodium berghei-infected red cells, which also have a high capacity for chloroquine accumulation. Pronase treatment of infected cells result only in a slight enhancement of total accumulation. The membrane-bound fraction of the drug is, however, increased while the fraction in the lysate is decreased. Membranes prepared from hypotonic lysis of normal or P. berghei-infected cells have similar capacities for chloroquine binding. These results show that the distribution of chloroquine in pronase-treated and malaria-infected cells are different and that pronase treatment of both normal and infected cells followed by lysis leads to availability of potential membrane binding sites. © 1980.Mahidol UniversityPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/0024-3205(80)90619-0