Khongjaroensakun N.Paisooksantivatana K.Santiwatana S.Tawonsawatruk T.Kusolthammarat K.Kadegasem P.Tangbubpha N.Chantaraamporn J.Chuansumrit A.Mahidol University2023-06-182023-06-182022-09-01Lab Medicine Vol.53 No.5 (2022) , E109-E11200075027https://repository.li.mahidol.ac.th/handle/20.500.14594/85572Objectives: To determine and compare the platelet growth factors in human platelet lysate (HPL) prepared from citrated whole blood, with final centrifugations at 4oC and 25oC. Methods: We collected specimens of citrated whole blood from 27 healthy volunteers. The platelet-rich plasma (PRP) was separated to prepare the HPL, which was further divided into 2 portions for the final centrifugation, at 4oC and 25oC, respectively. Platelet growth factors were measured and compared between the 2 groups. Results: All platelet growth factors were higher than those in PRP prepared from citrated whole blood. Moreover, the final centrifugation at 25oC resulted in noninferiority of platelet-growth-factor level. Conclusion: This study provided a simple method for small-volume of HPL preparation using only 10-15 mL of citrated whole blood. Further, the entire process of centrifugation can be performed at room temperature of 25oC, which is more applicable than lower temperatures for other laboratories.MedicineArticleSCOPUS10.1093/labmed/lmab1162-s2.0-851371371301943773035016203