Nuno ValeMaria João GouveiaMónica BotelhoBanchob SripaSutas SuttiprapaGabriel RinaldiPaula GomesPaul J. BrindleyJosé Manuel Correia da CostaUniversidade do PortoNational Health Institute Doutor Ricardo Jorge PortoKhon Kaen UniversityMahidol UniversityGeorge Washington UniversityUniversidad de la Republica Facultad de Medicina2018-10-192018-10-192013-12-01Parasitology International. Vol.62, No.6 (2013), 535-54218730329138357692-s2.0-84883274221https://repository.li.mahidol.ac.th/handle/20.500.14594/31831Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone. © 2013 Published by Elsevier Ireland Ltd.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.parint.2013.08.001