Atsushi YamanakaMeng Ling MoiTomohiko TakasakiIchiro KuraneMami MatsudaRyosuke SuzukiEiji KonishiMahidol UniversityOsaka UniversityNational Institute of Infectious DiseasesNagasaki UniversityKanagawa Prefectural Institute of Public Health2018-12-212019-03-142018-12-212019-03-142017-05-01Journal of Virological Methods. Vol.243, (2017), 164-17118790984016609342-s2.0-85014052708https://repository.li.mahidol.ac.th/handle/20.500.14594/42811© 2017 Elsevier B.V. The introduction of a foreign virus into an area may cause an outbreak, as with the Zika virus (ZIKV) outbreak in the Americas. Preparedness for handling a viral outbreak involves the development of tests for the serodiagnosis of foreign virus infections. We previously established a gene-based technology to generate some flaviviral antigens useful for functional antibody assays. The technology utilizes a Japanese encephalitis virus subgenomic replicon to generate single-round infectious particles (SRIPs) that possess designed surface antigens. In the present study, we successfully expanded the capacity of SRIPs to four human-pathogenic mosquito-borne flaviviruses that could potentially be introduced from endemic to non-endemic countries: ZIKV, Sepik virus, Wesselsbron virus, and Usutu virus. Flavivirus-crossreactive monoclonal antibodies dose-dependently neutralized these SRIPs. ZIKV-SRIPs also produced antibody-dose-dependent neutralization curves equivalent to those shown by authentic ZIKV particles using sera from a Zika fever patient. The faithful expression of designed surface antigens on SRIPs will allow their use in neutralization tests to diagnose foreign flaviviral infections.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/j.jviromet.2017.02.011