Jisnuson SvastiNapasri TalupphetMahidol University2018-06-012018-06-011979-03-27BBA - Protein Structure. Vol.577, No.1 (1979), 221-225000527952-s2.0-0018802443https://repository.li.mahidol.ac.th/handle/20.500.14594/13247By use of the neutral alkylating agent iodo [ 14 C 1 ] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis. © 1979.Mahidol UniversityMedicineArticleSCOPUS10.1016/0005-2795(79)90024-2