Panisa PornpienpakdeeRonnawich SinghasurasakPongsathorn ChaiyasapRath PichyangkuraRuengwit BunjongratSupachitra ChadchawanPatchra LimpanavechMahidol UniversityChulalongkorn University2018-09-242018-09-242010-05-01Scientia Horticulturae. Vol.124, No.4 (2010), 490-499030442382-s2.0-77950368804https://repository.li.mahidol.ac.th/handle/20.500.14594/28507The appropriate chitosan types and concentrations for enhancing the in vitro micropropagation of Dendrobium 'Eiskul' were studied using 70, 80 and 90% N-deacetylated polymeric (P-70, P-80 and P-90) and oligomeric (O-70, O-80 and O-90) forms of crab (Portunus pelagicus) chitosan. For the initial protocorm-like body (PLB) multiplication, the application of 10 mg/L of P-70 or 20 mg/L of P-90 was optimal, although 10 mg/L of P-80 and O-70 were also effective, and attained maximal PLB replication rates without increasing the detectable levels of somaclonal variation. However, during PLB-shoot induction, 10 or 20 mg/L of O-80 was the most appropriate chitosan and also induced further PLB formation. For plantlet regeneration, the addition of 10 mg/L of O-80 or P-80 gave the best quantity and quality, respectively, of plantlets. Finally, 20 mg/L of P-70 chitosan as a supplement during exflasking enhanced both the survival rate and the growth of the plantlets at one month after exflasking. Together, these data reveal a potentially beneficial and applicable protocol for commercial orchid micropropagation. © 2010 Elsevier B.V. All rights reserved.Mahidol UniversityAgricultural and Biological SciencesArticleSCOPUS10.1016/j.scienta.2010.02.008