C.M. PhechkrajangChutima (Matayatsuk)Phechkrajangชุติมา(มัธยัสถ์สุข)เพ็ชรกระจ่างMahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry2011-03-182021-03-242011-03-182021-03-242011-03-182010Mahidol University Journal of Pharmaceutical Science. Vol.37, No.1-2 (2010),1-70125-1570https://repository.li.mahidol.ac.th/handle/123456789/61404Some inorganic anions such as BF4-, CF3COO-, ClO4-, and H2PO4-, can affect the solubility of proteins and amino acids. Their effects were observed since 1888 by Fran Hofmeister. During the past decade, these ions have been used increasingly in reversed-phase high-performance liquid chromatography (HPLC), because of their abilities to improve the retention of basic substances in their protonated forms without changing a new column or mobile phase pH. Moreover, addition of these ions in mobile phase can improve theoretical plate number and tailing factor as well.engMahidol UniversityChaotropic agentChaotropic effectOriginal ArticleMahidol University. Faculty of Pharmacy