P. LearngarunsriP. ChaiyenT. SrikhirinW. VeerasaiS. DangtipMahidol UniversitySouth Carolina Commission on Higher Education2018-06-112018-06-112012-01-01Physics Procedia. Vol.32, (2012), 265-27118753892187538842-s2.0-85028805519https://repository.li.mahidol.ac.th/handle/20.500.14594/15193© 2012 Published by Elsevier B.V. Trends in disposable and handheld biosensors have called for miniaturized and planar electrodes in the place of conventional bulky ones. In this work, thin tin-doped indium oxide (ITO) film coated on glass were used as a based electrode in a three-electrode electrochemical system to follow some biochemical activities, such as NADH and phenol activity. The reference electrode is made on ITO layer by electro-deposition of thin nickel layer and silver layer, consecutively, followed by chlorination of silver surface by electrochemical potentiostatic method. The plain planar ITO-coated glasses were used as both counter electrode and working electrodes. Cyclic-voltammetry measurements; which follow an oxidation of 200 μM nicotinamide adenine dinucleotide (NADH) in 0.1 M KCl, show current peak of 8.5 μA. Another case was also to follow the NADH oxidation but in competition with its coupling activity with flavin mononucleotide (FMN). In this case, cyclic-voltammetry measurements were carried out of 200 μM NADH in 50 mM sodium phosphate; current peak of 1.3 μA was observed.Mahidol UniversityPhysics and AstronomyConference PaperSCOPUS10.1016/j.phpro.2012.03.553