Christopher G. JacobNguyen Thuy-NhienMayfong MayxayRichard J. MaudeHuynh Hong QuangBouasy HongvanthongViengxay VanisavethThang Ngo DucHuy RekolRob van der PluijmLorenz von SeidleinRick FairhurstFrançois NostenMd Amir HossainNaomi ParkScott GoodwinPascal RingwaldKeobouphaphone ChindavongsaPaul NewtonElizabeth AshleySonexay PhalivongRapeephan MaudeRithea LeangCheah HuchLe Thanh DongKim Tuyen NguyenTran Minh NhatTran Tinh HienHoa NguyenNicole ZdrojewskiSara CanavatiAbdullah Abu SayeedDidar UddinCaroline BuckeeCaterina I. FanelloMarie OnyambokoThomas PetoRupam TripuraChanaki AmaratungaAung Myint ThuGilles DelmasJordi LandierDaniel M. ParkerNguyen Hoang ChauDysoley LekSeila SuonJames CalleryPodjanee JittamalaBorimas HanboonkunupakarnSasithon PukrittayakameeAung Pyae PhyoFrank SmithuisKhin LinMyo ThantTin Maung HlaingParthasarathi SatpathiSanghamitra SatpathiPrativa K. BeheraAmar TripuraSubrata BaidyaNeena ValechaAnupkumar R. AnvikarAkhter Ul IslamAbul FaizChanon KunasolEleanor DruryMihir KekreMozam AliKatie LoveShavanthi RajatilekaAnna E. JeffreysKate RowlandsChristina S. HubbartMehul DhordaRanitha VongpromekNamfon KotananPhrutsamon WongnakJacob Almagro GarciaRichard D. PearsonCristina V. ArianiThanat ChookajornCinzia MalangoneT. NguyenJim StalkerBen JefferyJonathan KeatleyKimberly J. JohnsonDawn MuddymanXin Hui S. ChanJohn SillitoeRoberto AmatoVictoria SimpsonSonia GonçalvesKirk RockettNicholas P. DayArjen M. DondorpDominic P. KwiatkowskiOlivo MiottoRamathibodi HospitalFaculty of Tropical Medicine, Mahidol UniversityOxford University Clinical Research UnitSciences Economiques et Sociales de la Santé et Traitement de l'Information MédicaleThe Wellcome Centre for Human GeneticsMinistry of Health LaosUniversite de KinshasaNational Institute of Malariology, Parasitology and Entomology HanoiIspat General HospitalShoklo Malaria Research UnitHarvard T.H. Chan School of Public HealthOrganisation Mondiale de la SantéNational Institute of Malaria Research IndiaMahosot Hospital, LaoMahidol UniversityChittagong Medical College HospitalNuffield Department of MedicineUniversity of California, IrvineNational Institutes of Health (NIH)Wellcome Sanger InstituteMidnapore Medical CollegeVysnova Partners IncMyanmar Oxford Clinical Research UnitAsia Regional CentreRamu Upazila Health ComplexMalaria Research Group and Dev Care FoundationInstitute of MalariologyDefence Services Medical AcademyEntomologyAgartala Medical CollegeRoyal Society of ThailandInstitute of Malariology2022-08-042022-08-042021-01-01eLife. Vol.10, (2021)2050084X2-s2.0-85113798922https://repository.li.mahidol.ac.th/handle/20.500.14594/76341Background: National Malaria Control Programmes (NMCPs) currently make limited use of parasite genetic data. We have developed GenRe-Mekong, a platform for genetic surveillance of malaria in the Greater Mekong Subregion (GMS) that enables NMCPs to implement large-scale surveillance projects by integrating simple sample collection procedures in routine public health procedures. Methods: Samples from symptomatic patients are processed by SpotMalaria, a high-throughput system that produces a comprehensive set of genotypes comprising several drug resistance markers, species markers and a genomic barcode. GenRe-Mekong delivers Genetic Report Cards, a compendium of genotypes and phenotype predictions used to map prevalence of resistance to multiple drugs. Results: GenRe-Mekong has worked with NMCPs and research projects in eight countries, processing 9623 samples from clinical cases. Monitoring resistance markers has been valuable for tracking the rapid spread of parasites resistant to the dihydroartemisinin-piperaquine combination therapy. In Vietnam and Laos, GenRe-Mekong data have provided novel knowledge about the spread of these resistant strains into previously unaffected provinces, informing decision-making by NMCPs. Conclusions: GenRe-Mekong provides detailed knowledge about drug resistance at a local level, and facilitates data sharing at a regional level, enabling cross-border resistance monitoring and providing the public health community with valuable insights. The project provides a rich open data resource to benefit the entire malaria community.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyNeuroscienceArticleSCOPUS10.7554/ELIFE.62997