Varintip SrinonSunsiree MuangmanNithima ImyaemVeerachat MuangsombutNatalie R. Lazar AdlerEdouard E. GalyovSunee KorbsrisateMahidol UniversityUniversity of Leicester2018-10-192018-10-192013-08-01Journal of Microbiology. Vol.51, No.4 (2013), 522-52619763794122588732-s2.0-84883407644https://repository.li.mahidol.ac.th/handle/123456789/31890Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei. © 2013 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1007/s12275-013-2557-3