Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line

Benjamin SchmidKennie R. PrehnNatakarn NimsanorBlanca Irene Aldana GarciaUlla PoulsenIda JørringMikkel A. RasmussenChristian ClausenUlrike A. Mau-HolzmannSarayu RamakrishnaRavi MuddashettyRachel SteegKevin BrucePeter MackintoshAndreas EbnethBjørn HolstAlfredo Cabrera-SocorroJanssen Research & DevelopmentKøbenhavns UniversitetUniversität TübingenBioneer ASMahidol UniversityCenso BiotechnologiesUniversity of Trans-Disciplinary Health Sciences and Technology (TDU)Institute for Stem Cell Biology and Regenerative Medicine2020-01-272020-01-272019-01-01Stem Cell Research. Vol.34, (2019)18767753187350612-s2.0-85060108767https://repository.li.mahidol.ac.th/handle/20.500.14594/50404© 2019 The Authors Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-ε2, APOE-ε3 and APOE-ε4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-ε3/ε4 genotype to obtain isogenic APOE-ε2/ε2, APOE-ε3/ε3, APOE-ε4/ε4 lines as well as an APOE-knock-out line.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyGeneration of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out lineArticleSCOPUS10.1016/j.scr.2018.11.010