Sungsit Sungvornyothinสังสิทธิ์ สังวรโยธินMahidol University. Faculty of Tropical Medicine. Department of Medical Entomology2015-07-062021-08-172015-07-062021-08-172015-07-062014https://repository.li.mahidol.ac.th/handle/20.500.14594/63166Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 254.A double layer net (DLN) for sampling of malaria vectors was developed to compare the sampling efficacy of this method against the conventional method of human landing catch under semi-field conditions in a screenwalled corridor using laboratory reared Anopheles dirus KMK strain. A total of 1300 Anopheles dirus mosquitoes were used for the study and we found that the collection ratio between the double layer net and human landing catch was 1:2. The ratio of double layer net catches to those of the human landing catch increased with decreasing mosquito density. The collection rate was higher for the conventional method upon the release of the mosquito but gradually decreased with decreasing density. Mosquito species did not affect the ratio of double layer net to human landing catches since the trial with Aedes aegypti Aedes albopictus showed a consistent ratio. Two different colors of outer nets were used (black and white) to compare the sampling sensitivity between the two different colored materials. The results indicate that there was no difference in the number of host seeking mosquitoes caught with an equal ratio of number of catches. The double layer nets were used to prevent collectors from potentially infectious mosquito bites and are designed to catch host-seeking mosquitoes only. Although the sensitivity is half that of conventional method, these traps could be further improved.engMahidol UniversityAnopheline mosquitoesDouble layer netHuman landing catchProceeding Poster