Vanaporn WuthiekanunMichael D. SmithDavid A.B. DanceNicholas J. WhiteMahidol UniversityNuffield Department of Clinical MedicineLondon School of Hygiene & Tropical Medicine2018-07-042018-07-041995-01-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.89, No.1 (1995), 41-4318783503003592032-s2.0-0028926865https://repository.li.mahidol.ac.th/handle/20.500.14594/17330In order to optimize the recovery from soil of Pseudomonas pseudomallei, the cause of melioidosis, 3 selective broths were compared. A basal salt solution containing l-threonine (TBSS) performed significantly better than trypticase soy broth containing crystal violet and colistin 50 mg/L (CVC50), both in isolation rate and suppression of overgrowth of other organisms, but the addition of colistin to TBSS gave the best results overall. In a survey in north-eastern Thailand, P. pseudomallei was recovered from 114 (68%) of the 167 sites tested. A detailed study of a single rice farm showed that the isolation rate increased with depth of soil sample, and P. pseudomallei could still be isolated during the dry season, although only from moist soil in areas where other crops were cultivated and around the water source. © 1995 Oxford University Press.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/0035-9203(95)90651-7