Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen

Parima BoontanomSuradej SiripattanapipongMathirut MungthinPeerapan Tan-AriyaSaovanee LeelayoovaMahidol UniversityPhramongkutklao College of Medicine2018-09-242018-09-242010-03-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.2 (2010), 280-284012515622-s2.0-77956028306https://repository.li.mahidol.ac.th/handle/20.500.14594/29744A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).Mahidol UniversityMedicineImproved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimenArticleSCOPUS