Teewara PhongnimitrYuanyuan LiangKanokwan SrirattanaKanchana PanyawaiNucharin SripunyaChatchai TreetampinichRangsun ParnpaiSuranaree University of TechnologyMahidol University2018-10-192018-10-192013-11-01Animal Science Journal. Vol.84, No.11 (2013), 719-72517400929134439412-s2.0-84885953685https://repository.li.mahidol.ac.th/handle/20.500.14594/30958In this study, the effects of the addition of L-carnitine in in vitro maturation (IVM) medium for bovine oocytes on their nuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with 0.0, 0.3, 0.6 and 1.2mg/mL of L-carnitine (control, 0.3, 0.6 and 1.2 groups, respectively) and some of them were vitrified by Cryotop. Moreover, the effects of L-carnitine during in vitro fertilization (IVF) and in vitro culture (IVC) on the developmental potential and quality of IVF embryos were also examined. A significantly higher maturation rate of oocytes was obtained for 0.3 and 0.6mg/mL groups compared with the control (P<0.05). The blastocyst formation rate in the 0.6 group was significantly improved, whereas the rate in the 1.2 group was significantly decreased when compared with the control group (P<0.05). No significant difference was found in embryo development between the control and the L-carnitine group after oocyte vitrification. Supplementation of IVF and IVC media with L-carnitine had no effect on development to the blastocyst stage of IVM oocytes treated with 0.6mg/mL L-carnitine. In conclusion, the supplementation of L-carnitine during IVM of bovine oocytes improved their nuclear maturation and subsequent embryo development after IVF, but when they were vitrified the improving effects were neutralized. © 2013 Japanese Society of Animal Science.Mahidol UniversityAgricultural and Biological SciencesArticleSCOPUS10.1111/asj.12067