Run‐Tao ‐T HeSongli WangRobert AndersonBruce L. InnisAnanda NisalakWipawee UsawattanakulSiripen Kalayanarooj AndUniversity of CalgaryArmed Forces Research Institute of Medical Sciences, ThailandMahidol UniversityDivision of Infectious DiseasesWalter Reed Army Institute of Research2018-07-042018-07-041995-01-01Journal of Medical Virology. Vol.45, No.4 (1995), 451-46110969071014666152-s2.0-0028921203https://repository.li.mahidol.ac.th/handle/123456789/17329Epidemiological data strongly implicate a role for the host humoral immune response in both protection against and exacerbation of dengue virus‐caused disease. In an effort to characterize elements of the normal human immune response against dengue virus we have addressed the issue of antibody‐mediated neutralization of dengue virus. We show here the ability of both mouse monoclonal antibody 3H5 and human anti‐dengue neutralizing sera to block binding of dengue‐2 virus to monkey kidney (Vero) cells. Since Vero cells possess virus receptors but not Fc receptors we conclude that the major effect of host neutralizing antibodies is to block virus attachment to Vero cell dengue virus receptors. Analysis of 61 patient antisera yielded good correlation (Pearson's coefficient = 0.90; P < 0.001) between neutralizing activity and ability to block virus‐cell attachment suggesting that antibody‐mediated neutralization of dengue virus occurs primarily extracellularly and less by a postat‐tachment mechanism as has been described for certain other viruses. © 1995 Wiley‐Liss, inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley CompanyMahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1002/jmv.1890450417