Shirley Yi Fen HiiSarunporn TandhavanantRungnapa PhunpangPeeraya EkchariyawatNatnaree SaipromClaire ChewapreechaRathanin SengEkkachai ThiansukhonChumpol MorakotNarongchai SangsaSunee ChayangsuSomchai ChuananontKittisak TanwisaidWirayut SilakunNoppol BuasiSeksan ChaisuksantTanin HompleumPloenchan ChetchotisakdNicholas P.J. DayWasun ChantratitaGanjana LertmemongkolchaiT. Eoin WestNarisara ChantratitaRamathibodi HospitalFaculty of Tropical Medicine, Mahidol UniversityUdon Thani Center HospitalFaculty of Medicine, Khon Kaen UniversitySurin HospitalKhon Kaen UniversityKhon Kaen Regional HospitalMahidol UniversityNuffield Department of MedicineKing Mongkut's University of Technology ThonburiWellcome Sanger InstituteHarborview Medical CenterBuriram HospitalSisaket HospitalRoi Et HospitalNakhon Phanom HospitalMukdahan Hospital2022-08-042022-08-042021-05-01Antimicrobial Agents and Chemotherapy. Vol.65, No.5 (2021)10986596006648042-s2.0-85105098806https://repository.li.mahidol.ac.th/handle/123456789/78246Melioidosis is an often fatal infection in tropical regions caused by an environmental bacterium, Burkholderia pseudomallei. Current recommended melioidosis treatment requires intravenous β-lactam antibiotics such as ceftazidime (CAZ), meropenem (MEM), or amoxicillin-clavulanic acid (AMC) and oral trimethoprim-sulfamethoxazole. Emerging antibiotic resistance could lead to therapy failure and high mortality. We performed a prospective multicenter study in northeast Thailand from 2015 to 2018 to evaluate antibiotic susceptibility and characterize β-lactam resistance in clinical B. pseudomallei isolates. A collection of 1,317 B. pseudomallei isolates from patients with primary and relapse infections were evaluated for susceptibility to CAZ, imipenem (IPM), MEM, and AMC. β-Lactam-resistant isolates were confirmed by the broth microdilution method and characterized by whole-genome sequence analysis, penA expression, and β-lactamase activity. The resistant phenotype was verified via penA mutagenesis. All primary isolates were IPM susceptible, but we observed two CAZ-resistant isolates and one CAZ-intermediate isolate, two MEMCitation less-susceptible isolates, and one AMC-resistant and two AMC-intermediate isolates. One of 13 relapse isolates was resistant to both CAZ and AMC. Two isolates were MEM less susceptible. Strains DR10212A (primary) and DR50054E (relapse) were multidrug resistant. Genomic and mutagenesis analyses supplemented with gene expression and β-lactamase analyses demonstrated that the CAZ-resistant phenotype was caused by PenA variants: P167S (n = 2) and penA amplification (n = 1). Despite the high mortality rate in melioidosis, our study revealed that B. pseudomallei isolates had a low frequency of β-lactam resistance caused by penA alterations. Clinical data suggest that resistant variants may emerge in patients during antibiotic therapy and may be associated with a poor response to treatment.Mahidol UniversityMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1128/AAC.02230-20