Boonyasuppayakorn S.Saelee T.Huynh T.N.T.Hairani R.Hengphasatporn K.Loeanurit N.Cao V.Vibulakhaophan V.Siripitakpong P.Kaur P.Chu J.J.H.Tunghirun C.Choksupmanee O.Chimnaronk S.Shigeta Y.Rungrotmongkol T.Chavasiri W.Mahidol University2023-05-242023-05-242023-12-01Scientific Reports Vol.13 No.1 (2023)https://repository.li.mahidol.ac.th/handle/20.500.14594/82797Dengue and Zika viruses are mosquito-borne flaviviruses burdening millions every year with hemorrhagic fever and neurological symptoms. Baicalein was previously reported as a potential anti-flaviviral candidate and halogenation of flavones and flavanones potentiated their antiviral efficacies. Here, we reported that a chemically modified 8-bromobaicalein effectively inhibited all dengue serotypes and Zika viruses at 0.66–0.88 micromolar in cell-based system. The compound bound to dengue serotype 2 conserved pocket and inhibited the dengue RdRp activity with 6.93 fold more than the original baicalein. Moreover, the compound was mildly toxic against infant and adult C57BL/6 mice despite administering continuously for 7 days. Therefore, the 8-bromobaicalein should be investigated further in pharmacokinetics and efficacy in an animal model.MultidisciplinaryArticleSCOPUS10.1038/s41598-023-32049-x2-s2.0-851509910332045232236966240