Poom Adisakwattanaภูมิ อดิศักดิ์วัฒนาSupaporn Nuamtanongสุภาพร น่วมทะนงTeera Kusolsukธีระ กุศลสุขMontri Chairojมนตรี ไชยโรจน์Pa-Thai YenchitsomanasUrai Chaisriอุไร ไชยศรีMahidol University. Faculty of Tropical Medicine. Department of HelminthologyMahidol University. Faculty of Tropical Medicine. Hospital for Tropical Diseases. Radiology unit (X-ray)Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology2015-09-252021-08-172015-09-252021-08-172015-09-252013https://repository.li.mahidol.ac.th/handle/20.500.14594/63190Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.133.Background: Helminths use various mechanisms to avoid host immunity and protect themselves from being eliminated. Despite evading host immune responses, immunosuppression and regulation mechanisms elicit functions that diminish the adverse effects of unrelated inflammatory diseases. Objective: We investigated whether helminthic infections can ameliorate inflammatory diseases. Methods: Mice were infected with Trichinella papuae and then subjected to induced colitis through the oral administration of dextran sulfate sodium (DSS). Macroscopic and microscopic examinations measured weight loss, stool consistency, gross bleeding, colon length, and tissue inflammation. In addition, cytokine expression was observed in colon tissue by SYBR real-time RT -PCR to investigate the Th1, Th2, and regulatory cytokines. Result: The results showed that T. papuae infection decreased the severity of DSS-induced colitis, including weight loss, bloody diarrhea, shortening of colon, and colon tissue damage in mice (p < 0.05). The expression level of IL-4 was high in the colons of DSS-treated mice without helminthic infection, while infected mice with DSS treatment had lower IL-4 levels (p 0.05). Uninfected DSS-treated mice failed to produce IL-10 mRNA in colon tissue, which may cause more severe colitis. In contrast, prior to T. papuae infection DSS-treated mice had IL-10 levels in the colon significant lower than the normal and infected control groups. Conclusion: Our data provide the evidence that prior T. papuae infection can ameliorate DSS-induced colitis in mice and may be considered for a novel therapeutic strategy against immunological diseases in the future.engMahidol UniversityColitisInflammatory bowel diseasesProceeding Poster