Pluangnooch P.Phayangkhe C.Soontrapa K.Mahidol University2023-12-152023-12-152023-12-01Asian Pacific Journal of Allergy and Immunology Vol.41 No.4 (2023) , 401-4060125877Xhttps://repository.li.mahidol.ac.th/handle/20.500.14594/91468Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares. Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry. Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules. Conclusion: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.MedicineArticleSCOPUS10.12932/ap-140720-09132-s2.0-851786686102228869433274953