Buranrat B.Junking M.Mahidol University2023-06-182023-06-182022-01-01Asian Pacific Journal of Tropical Biomedicine Vol.12 No.1 (2022) , 39-4622211691https://repository.li.mahidol.ac.th/handle/20.500.14594/83930Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G 0 / G 1 phase and cell arrest at the G 2 /M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.4103/2221-1691.3332112-s2.0-85123400719