Jantana KampeeraPatiya PasakonChanpen KaruwanNarong ArunrutAssawapong SappatSarawut SirithammajakNipaphorn DechokiattawanThitinun SumranwanichParin ChaivisuthangkuraPuey OunjaiSurang ChankhamhaengdechaAnurat WisitsoraatAdisorn TuantranontWansika KiatpathomchaiThailand Ministry of EducationMahidol UniversityThailand National Center for Genetic Engineering and BiotechnologyThailand National Science and Technology Development AgencySrinakharinwirot University2020-01-272020-01-272019-05-01Biosensors and Bioelectronics. Vol.132, (2019), 271-27818734235095656632-s2.0-85062811982https://repository.li.mahidol.ac.th/handle/123456789/50190© 2019 Elsevier B.V. Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryEngineeringArticleSCOPUS10.1016/j.bios.2019.02.060