Vithoon ViyanantSuchart UpathamRojana KeawjamNitaya Sritong2025-02-032025-02-03199020251990Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1990https://repository.li.mahidol.ac.th/handle/20.500.14594/103333Environmental Biology (Mahidol University 1990)Opisthorchis viverrini and Fasciola gigantica are parasites residing in the liver of man and animals. The fluke, O. viverrini is common in fish-eating animals including man, whereas F. gigantica is found in herbivorous animals and occasionally in human. Isoenzymes of the adult's O. viverrini and F. gigantica were analyzed by isoelectric focusing in polyacrylamide gel at the same electrophoresis condition. The eleven enzymes studied were: acid phosphatase, alkaline phosphatase, esterase, hexokinase, lactate dehydrogenase, glutamate dehydrogenase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase and 6-phosphogluconate dehydrogenase. Among those enzymes examined, acid phosphatase, phosphoglucoisomerase, phosphoglucomutase and malate dehydrogenase showed high enzyme activities, whereas alkaline phosphatase, esterase, lactate dehydrogenase, hexokinase and 6-phosphogluconate dehydrogenase showed low enzyme activities. All of the eleven enzymes were found in F. gigantica, but four of them, namely, alkaline phosphatase, esterase, glutamate dehydrogenase and lactate dehydrogenase were not detected in O. viverrini. Genotypic differences between the two species were clearly distinguished by three enzymatic systems, namely, acid phosphatase, phosphoglucoisomerase and phosphoglucomutase. Among the eleven enzymes, phosphoglucomutase isoenzyme variation was found in O. viverrini. This work indicates that O. viverrini and F. gigantica can be distinguished by their protein and isoenzyme patterns besides from their morphological differences.vi, 93 leaves : ill. (some col.)application/pdfengผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้าFasciolaOpisthorchisIsoenzymes -- AnalysisMaster ThesisMahidol University