Sumet KongkiatpaiboonVichien KeeratinijakalWandee GritsanapanMahidol UniversityKasetsart University2018-06-112018-06-112012-11-29Natural Product Communications. Vol.7, No.10 (2012), 1277-1278155594751934578X2-s2.0-84869774131https://repository.li.mahidol.ac.th/handle/20.500.14594/13367A HPLC-UV method was developed and validated for determination of stemokerrine and oxystemokerrine in Stemona kerrii roots. The chromatographic separation was performed on a Hypersil BDS C 18 -column eluted with methanol: 50 mM ammonium acetate solution using a gradient system with a flow rate of 1 mL/min and detection at 300 nm. Stemokerrine and oxystemokerrine showed a linear relationship within the range of 0.5-100 μg/mL. The method was shown to be precise with a RSD < 2%. The average percent recovery of stemokerrine was 101.6% and for oxystemokerrine 99.5%. Two samples of S. kerrii were analyzed and the average contents of stemokerrine and oxystemokerrine were 0.2 and 0.05%, w/w, respectively. The present work will provide a useful standardization method for S. kerrii raw materials for further pharmaceutical development.Mahidol UniversityAgricultural and Biological SciencesMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS