Jiahao WuRattikan ChantiwasAlborz AmirsadeghiSteven A. SoperSunggook ParkLouisiana State UniversityMahidol University2018-05-032018-05-032011-09-07Lab on a Chip. Vol.11, No.17 (2011), 2984-298914730189147301972-s2.0-80051651069https://repository.li.mahidol.ac.th/handle/20.500.14594/11475Development of all polymer-based nanofluidic devices using replication technologies, which is a prerequisite for providing devices for a larger user base, is hampered by undesired substrate deformation associated with the replication of multi-scale structures. Therefore, most nanofluidic devices have been fabricated in glass-like substrates or in a polymer resist layer coated on a substrate. This letter presents a rapid, high fidelity direct imprinting process to build polymer nanofluidic devices in a single step. Undesired substrate deformation during imprinting was significantly reduced through the use of a polymer stamp made from a UV-curable resin. The integrity of the enclosed all polymer-based nanofluidic system was verified by a fluorescein filling experiment and translocation/stretching of λ-DNA molecules through the nanochannels. It was also found that the funnel-like design of the nanochannel inlet significantly improved the entrance of DNA molecules into nanochannels compared to an abrupt nanochannel/microfluidic network interface. © 2011 The Royal Society of Chemistry.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringChemistryEngineeringArticleSCOPUS10.1039/c1lc20294d