Supaporn SuparakVeerachat MuangsombutDonporn RiyapaJoanne M. StevensMark P. StevensGanjana LertmemongkolchaiSunee KorbsrisateMahidol UniversityKhon Kaen UniversityUniversity of Edinburgh, Roslin Institute2018-05-032018-05-032011-11-01Microbes and Infection. Vol.13, No.12-13 (2011), 1006-10111769714X128645792-s2.0-80053386594https://repository.li.mahidol.ac.th/handle/20.500.14594/11973Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion. © 2011 Institut Pasteur.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.micinf.2011.06.007