Thanyaporn DechtawewatAtchara PaemaneeSittiruk RoytrakulPucharee SongprakhonThawornchai LimjindapornPa thai YenchitsomanusSawanan SaitornuangChunya PuttikhuntWatchara KasinrerkPrida MalasitSansanee NoisakranMahidol UniversityThailand National Center for Genetic Engineering and BiotechnologyChiang Mai University2018-12-112019-03-142018-12-112019-03-142016-09-01Biochimica et Biophysica Acta - Proteins and Proteomics. Vol.1864, No.9 (2016), 1270-128018781454157096392-s2.0-84966716417https://repository.li.mahidol.ac.th/handle/20.500.14594/42974© 2016 Elsevier B.V. Dengue virus (DENV) infection is a leading cause of the mosquito-borne infectious diseases that affect humans worldwide. Virus–host interactions appear to play significant roles in DENV replication and the pathogenesis of DENV infection. Nonstructural protein 1 (NS1) of DENV is likely involved in these processes; however, its associations with host cell proteins in DENV infection remain unclear. In this study, we used a combination of techniques (immunoprecipitation, in-solution trypsin digestion, and LC–MS/MS) to identify the host cell proteins that interact with cell-associated NS1 in an in vitro model of DENV infection in the human hepatocyte HepG2 cell line. Thirty-six novel host cell proteins were identified as potential DENV NS1-interacting partners. A large number of these proteins had characteristic binding or catalytic activities, and were involved in cellular metabolism. Coimmunoprecipitation and colocalization assays confirmed the interactions of DENV NS1 and human NIMA-related kinase 2 (NEK2), thousand and one amino acid protein kinase 1 (TAO1), and component of oligomeric Golgi complex 1 (COG1) proteins in virus-infected cells. This study reports a novel set of DENV NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryArticleSCOPUS10.1016/j.bbapap.2016.04.008