Juree CharoenteeraboonKasem NithipatikomWilliam B. CampbellPawinee PiyachaturawatPrapon WilairatPornpimol RongnoparutMahidol UniversityMedical College of Wisconsin2018-06-212018-06-212005-05-16European Journal of Pharmacology. Vol.515, No.1-3 (2005), 43-46001429992-s2.0-20344362658https://repository.li.mahidol.ac.th/handle/20.500.14594/17146In animal the plasma cholesterol-lowering activity of 2,4,6- trihydroxyacetophenone (THA) is due to enhanced cholesterol 7α-hydroxylase (CYP7A1) activity. We have examined the effect of THA on CYP7A1 activity and mRNA level in HepG2 cells. THA stimulated CYP7A1 activity in a concentration- and time-dependent manner. After exposure for 24 h, 1 μM THA induced CYP7A1 activity 160 ± 8% and mRNA level 166 ± 21% (mean ± S.E.M.) of control. Moreover THA antagonized the inhibitory regulation of chenodeoxycholic acid on CYP7A1 mRNA expression. These results indicated that THA increases CYP7A1 activity in human HepG2 cells by stimulating mRNA transcription. © 2005 Elsevier B.V. All rights reserved.Mahidol UniversityNeurosciencePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/j.ejphar.2005.03.039