Pannamas ManeekanAkanitt JittmittraphapPornsawan LeaungwutiwongJaranit KaewkangwalNatthanej LuplertlopMahidol University2018-09-132018-09-132009-11-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1254-1258012515622-s2.0-76749114335https://repository.li.mahidol.ac.th/handle/20.500.14594/27864This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.Mahidol UniversityMedicineArticleSCOPUS