S. DhammachatK. SomkidS. PiboonpocanunO. ReamtongP. PacharnC. BunnagM. NakanoWisuwat SongnuanNiigata UniversityMahidol UniversityFaculty of Medicine, Siriraj Hospital, Mahidol University2020-01-272020-01-272019-01-01European Annals of Allergy and Clinical Immunology. Vol.51, No.4 (2019), 174-185176414892-s2.0-85069889544https://repository.li.mahidol.ac.th/handle/20.500.14594/52256© 2019, EDRA S.p.A. All rights reserved. Group 1 grass pollen allergens, or beta-expansins, are the most important major allergens from tropical/subtropical grasses. This study aimed to investigate the sequence similarity and immunoreactivity of group 1 allergens from para grass (Urochloa mutica). Three isoforms (Uro m 1.01, Uro m 1.02, and Uro m 1.03) were cloned from cDNA of para grass pollen. The acidic-neutral isoforms rUro m 1.01 and rUro m 1.02 could effectively inhibited beta-expansins in pollen extract of Bermuda and Johnson grasses, suggesting that these isoforms could be major cross-reacting allergens among these grasses. In contrast, the basic isoform rUro m 1.03 had limited IgE reactivity. Thus, group 1 allergens both acidic-neutral and basic isoforms could have markedly different IgE reactivity.Mahidol UniversityMedicineArticleSCOPUS10.23822/EurAnnACI.1764-1489.95