Tepmanas Bupha-IntrJonggonnee WattanapermpoolJames R. PeñaBeata M. WolskaR. J. SolaroUniversity of Illinois College of MedicineMahidol University2018-08-242018-08-242007-02-01American Journal of Physiology - Regulatory Integrative and Comparative Physiology. Vol.292, No.2 (2007)15221490036361192-s2.0-33846815376https://repository.li.mahidol.ac.th/handle/20.500.14594/24253Compared to sham-operated controls, myofilaments from hearts of ovariectomized (OVX) rats demonstrate an increase in Ca2+sensitivity with no change in maximum tension (Wattanapermpool J and Reiser PJ. Am J Physiol 277: H467-H473, 1999). To test the significance of this modification in intact cells, we compared intracellular Ca2+transients and shortening of ventricular myocytes isolated from sham and 10-wk OVX rats. There was a decrease in the peak Ca2+transient with prolonged 50% decay time in OVX cardiac myocytes without changes in the resting intracellular Ca2+concentration. Percent cell shortening was also depressed, and relaxation was prolonged in cardiac myocytes from OVX rats compared with shams. Ovariectomy induced a sensitization of the myofilaments to Ca2+. Hypercapnic acidosis suppressed the shortening of OVX myocytes to a lesser extent than that detected in shams. Moreover, a larger compensatory increase in %cell shortening was obtained in OVX myocytes during prolonged acidosis. The elevated compensation in cell shortening was related to a higher amount of increase in the amplitude of the Ca2+transient in OVX myocytes. However, these differences in Ca2+transients and %cell shortening were no longer evident in the presence of 1 μM cariporide, a specific inhibitor of Na+/H+exchanger type 1 (NHE1). Our results indicate that deprivation of female sex hormones modulates the intracellular Ca2+concentration in cardiac myocytes, possibly via an increased NHE1activity, which may act in concert with Ca2+hypersensitivity of myofilament activation as a determinant of sex differences in cardiac function. Copyright © 2007 the American Physiological Society.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1152/ajpregu.00376.2006