R. MahanondaN. Sa-Ard-IamO. CharatkulangkunA. PromsudthiR. E. SchifferleK. YongvanichitS. PichyangkulChulalongkorn UniversityMahidol UniversityUniversity at Buffalo, State University of New YorkArmed Forces Research Institute of Medical Sciences, Thailand2018-07-242018-07-242004-01-01Journal of Dental Research. Vol.83, No.7 (2004), 540-545002203452-s2.0-4143098465https://repository.li.mahidol.ac.th/handle/20.500.14594/21304In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and γδ T-cells were analyzed by flow cytometry, and the production of interleukin-1β and prostaglandin E2was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and γδ T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.Mahidol UniversityDentistryArticleSCOPUS10.1177/154405910408300706