Srinarawat W.Hemstapat R.Tawonsawatruk T.Patikarnmonthon N.Hamachi I.Ojida A.Wongkongkatep J.Mahidol University2023-06-182023-06-182022-09-20Analyst Vol.147 No.21 (2022) , 4910-491800032654https://repository.li.mahidol.ac.th/handle/20.500.14594/83604Calcium pyrophosphate deposition disease, previously known as pseudogout, is a type of chronic and painful joint arthropathy. Accurate identification of calcium pyrophosphate dihydrate (CPPD) single crystals is crucial for determining the best course of treatment. In this study, a two-step method involving alizarin red S (ARS) and a xanthene dipicolylamine ZnII (XDZ) complex was employed for the identification of CPPD single crystals in both triclinic and monoclinic forms using a fluorescence microscope and a microplate reader. The accurate identification method proposed in this study has the potential to advance the diagnosis and treatment of patients suffering from painful gouty arthritis.Biochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1039/d2an01257j2-s2.0-851404888671364552836172837