Jee Young KwonSeung Young LeePreeyaporn KoedrithJong Yun LeeKyoung Min KimJae Min OhSung Ik YangMeyoung Kon KimJong Kwon LeeJayoung JeongEun Ho MaengBeam Jun LeeYoung Rok SeoDongguk University, SeoulKyung Hee UniversityKorea Testing and Research InstituteChungbuk National UniversityMahidol University. Faculty of Environment and Resource StudiesYonsei UniversityKorea University, College of MedicineMinistry of Food and Drug Safety (MFDS)2018-11-092018-11-092014-02-01Mutation Research - Genetic Toxicology and Environmental Mutagenesis. Vol.761, (2014), 1-918793592138357182-s2.0-84893743466https://repository.li.mahidol.ac.th/handle/20.500.14594/33312The industrial application of nanotechnology, particularly using zinc oxide (ZnO), has grown rapidly, including products such as cosmetics, food, rubber, paints, and plastics. However, despite increasing population exposure to ZnO, its potential genotoxicity remains controversial. The biological effects of nanoparticles depend on their physicochemical properties. Preparations with well-defined physico-chemical properties and standardized test methods are required for assessing the genotoxicity of nanoparticles. In this study, we have evaluated the genotoxicity of four kinds of ZnO nanoparticles: 20. nm and 70. nm size, positively or negatively charged. Four different genotoxicity tests (bacterial mutagenicity assay, in vitro chromosomal aberration test, in vivo comet assay, and in vivo micronucleus test, were conducted, following Organization for Economic Cooperation and Development (OECD) test guidelines with good laboratory practice (GLP) procedures. No statistically significant differences from the solvent controls were observed. These results suggest that surface-modified ZnO nanoparticles do not induce genotoxicity in in vitro or in vivo test systems. © 2014 Elsevier B.V.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEnvironmental ScienceArticleSCOPUS10.1016/j.mrgentox.2014.01.005