Panat AnuracpreedaChaitip WanichanonPrasert SobhonMahidol University2018-09-132018-09-132009-06-01Experimental Parasitology. Vol.122, No.2 (2009), 75-8310902449001448942-s2.0-67349188005https://repository.li.mahidol.ac.th/handle/20.500.14594/27698Specific monoclonal antibody (MoAb) to 28.5 kDa tegumental antigen (TA) was used to localize this antigen in the tissues of metacercariae, newly excysted juvenile (NEJ), 1, 3, 5, and 7-week-old juveniles of Fasciola gigantica by using indirect immunofluorescence, immunoperoxidase and immunogold techniques. Both indirect immunofluorescence and immunoperoxidase detections showed that this antigen was concentrated in the tegument particularly in its outer rim, tegumental cells and their processes as well as epithelial linings of the oral sucker. Unlike adult F. gigantica, it was not detected in spermatogenic cells in the testes, cells of Mehlis'gland, oocytes within the ovary, and ovum within the egg of parasites. At the ultrastructural level, the immunogold labeling showed deposit of gold particles specifically in G2tegumental granules and on the surface membrane. Thus, this 28.5 kDa antigen is expressed in the tegument and associated structures of juvenile parasites, and it could be a major component of the G2granules which are shown to fuse with the surface membrane and contribute material to replace the casted-off membrane. This process is the replenishment and turnover of the surface membrane to prevent the attachment of the host immune effector cells. © 2009 Elsevier Inc. All rights reserved.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1016/j.exppara.2009.03.007