Chalermporn OngvarrasoponeEkapol ChomchaySakol PanyimMahidol University2018-09-242018-09-242010-10-01Antiviral Research. Vol.88, No.1 (2010), 116-11818729096016635422-s2.0-77956928010https://repository.li.mahidol.ac.th/handle/20.500.14594/29187PmRab7 is a Penaeus monodon small GTPase protein possibly involved in replication of several shrimp viruses. In this study RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PmRab7 gene (dsRNA-PmRab7) was employed to silence the expression of PmRab7 to investigate the inhibitory effect on Laem-Singh virus (LSNV) replication. Injection of dsRNA-PmRab7 24. h before challenge with the virus resulted in a drastic decrease of PmRab7 mRNA and complete inhibition of LSNV replication at 3 days post-challenge. In a therapeutic mode, shrimp injected with dsRNA-PmRab7 1 day but not at 3 or 5 days post-LSNV challenge resulted in inhibition of LSNV replication. These results pave the way to use dsRNA-PmRab7 to prevent or cure LSNV infection in shrimp. © 2010 Elsevier B.V.Mahidol UniversityImmunology and MicrobiologyPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/j.antiviral.2010.06.013