N. LindegårdhA. M. DondorpP. SinghasivanonN. J. WhiteN. P J DayMahidol UniversityNuffield Department of Clinical Medicine2018-08-242018-08-242007-09-21Journal of Pharmaceutical and Biomedical Analysis. Vol.45, No.1 (2007), 149-153073170852-s2.0-34548474579https://repository.li.mahidol.ac.th/handle/20.500.14594/24336A simple and rapid liquid chromatographic-mass spectrometric assay for the evaluation of artesunate in vials for injection has been developed and validated. The content of each vial was dissolved in 3.0 mL of methanol using a SGE analytical syringe (1.0 mL). Each sample was diluted to a theoretical concentration of 1000 ng/mL and analysed in triplicate. Three replicates of calibration standards at concentrations 500, 1000 and 1500 ng/mL were used to construct a calibration curve. Artesunate was analysed by liquid chromatography with atmospheric pressure chemical ionisation (APCI) mass spectrometric (MS) detection on a Hypersil Gold column (100 mm × 4.6 mm) using a mobile phase containing methanol-ammonium acetate 10 mM pH 5.3 (70:30, v/v) at a flow rate of 1 mL/min. The assay was implemented for the analysis of artesunate for injection purchased from Guilin Pharmaceutical Company in China. © 2007 Elsevier B.V. All rights reserved.Mahidol UniversityChemistryPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1016/j.jpba.2007.04.030