Chariya KaewsanehaKulachart JangpatarapongsaTienrat TangchaikeereeDuangporn PolpanichPramuan TangboriboonratMahidol UniversityThailand National Nanotechnology Center2018-11-092018-11-092014-01-01Journal of Biomaterials Applications. Vol.29, No.5 (2014), 761-76815308022088532822-s2.0-84908878845https://repository.li.mahidol.ac.th/handle/20.500.14594/33895© The Author(s) 2014. Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass through cell membrane within 2 h for K562 cells and 3 h for HeLa and Hep G2 cells and then confine inside cytoplasm of all types of tested cells for at least 24 h. Therefore, the synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate would potentially be used as cell tracking in theranostic applications.Mahidol UniversityEngineeringMaterials ScienceMedicineArticleSCOPUS10.1177/0885328214540349