Saovanee DharmsthitiSudaporn LuchaiMahidol University2018-07-042018-07-041998-01-01Journal of Fermentation and Bioengineering. Vol.86, No.3 (1998), 335-3370922338X2-s2.0-0031690949https://repository.li.mahidol.ac.th/handle/20.500.14594/18313Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13:163-166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na+) or IRC50 (H+) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (≈25 to 30°C) or at 4°C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1016/S0922-338X(98)80140-2