Luk-In S.Leepiyasakulchai C.Saelee C.Keeratichamroen A.Srisangwan N.Ponprachanuvut P.Chammari K.Chatsuwan T.Wannigama D.L.Shein A.M.S.Kueakulpattana N.Srisakul S.Sranacharoenpong K.Mahidol University2024-11-232024-11-232024-12-01Scientific Reports Vol.14 No.1 (2024)https://repository.li.mahidol.ac.th/handle/20.500.14594/102125Constipation poses a significant health concern affecting individuals of varying ages and geographic locations worldwide. While the impacts of numerous probiotics on constipation are well-characterized, there has been limited assessment of the potential prebiotic effects of resistant starches. We therefore conducted a randomized, double-blind and placebo-controlled, clinical trial of resistant starch type 3 (RS-3) in Thai adults with self-reported chronic constipation. The effects of these mixed natural starch fibers on beneficial gut bacteria, bowel movements and stool consistency were evaluated after 6- and 12-week periods. Regardless of subject age, consumption of RS-3 compared to placebo resulted in significant improvements in gut health by dramatically increasing levels of beneficial bacteria (Bifidobacterium, Prevotella, Akkermansia and Megamonas) in the gut and relieving constipation. RS-3 consumption was associated with a significantly increased frequency of bowel movements, with subjects reporting these as healthy stools. Our findings provide important insights into the therapeutic advantages of RS-3 for constipation, and propose RS-3 as a feasible alternative strategy for management of constipation.MultidisciplinaryArticleSCOPUS10.1038/s41598-024-79465-12-s2.0-8520922873520452322