Phomai K.Supharoek S.a.Vichapong J.Grudpan K.Ponhong K.Mahidol University2023-05-192023-05-192023-01-15Talanta Vol.252 (2023)00399140https://repository.li.mahidol.ac.th/handle/20.500.14594/81756Iron-tannic nanoparticles were used as a new adsorbent for dispersive solid phase extraction (DSPE) synergized with cloud point extraction (CPE) to enrich four tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) prior to high-performance liquid chromatographic determination. DSPE and CPE were performed simultaneously in a one-pot co-extraction to form iron-tannic nanoparticles in-situ and pre-concentrate the tetracyclines. The parameters affecting the extraction efficiency were investigated. Using the optimal parameters, linear calibrations ranging from 2.63 to 1000 ng mL−1 were obtained, with determination coefficients greater than 0.996. The limit of detection was found to be 1.06–3.19 ng mL−1, while the limit of quantification was 2.63–10.65 ng mL−1. Precision was expressed as a relative standard deviation of less than 10%. The residues of the four tetracyclines in milk, eggs, honey, chicken liver, and chicken kidney samples were determined by the proposed method. The recoveries ranged from 79.3 to 107.1%. The results indicated that the proposed method was an alternative method for the extraction and pre-concentration of tetracyclines with high extraction and enrichment efficiency. In addition, it promoted rapidity and environmental friendliness.ChemistryArticleSCOPUS10.1016/j.talanta.2022.1238522-s2.0-8513660471936041315