Apinya PumpuangNarisara ChantratitaChanthiwa WikraiphatNatnaree SaipromNicholas P J DaySharon J. PeacockVanaporn WuthiekanunMahidol University2018-05-032018-05-032011-10-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.10 (2011), 598-60018783503003592032-s2.0-80052967899https://repository.li.mahidol.ac.th/handle/20.500.14594/11981Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 7 cells/ml and 1.4×10 5 cells/ml, respectively. A colony count on agar of 1.0×10 6 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. © 2011 Royal Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.trstmh.2011.06.004